OUR BEST FRIENDS

The Sergei Foundation

shelterpet_logo

The Animal Rescue Site

B-more Dog

aldflogo

Pinups for Pitbulls

philadoptables

TFPF_Logo

Mid Atlantic Pug Rescue

Our Pack, Inc.

Maine Coonhound Rescue

Saving Shelter Pets, Inc.

mabb

LD Logo Color

Tag: science

Could a DNA test prove dog’s innocence?

Up to now, DNA testing on dogs has been used mostly to satisfy owner curiosity over what breeds are in their mutt, or by apartment managers who want to identify dogs whose owners didn’t pick up after them.

Now comes a chance to put it to more noble use. (Cue up the “Law & Order” theme.)

jebThe owners of a Belgian Malinois accused of killing a neighbor dog say a DNA test could clear their dog of a murder rap.

A district judge in Michigan ordered Jeb, the Belgian Malinois, to be euthanized after hearing the evidence against him on Sept. 19.

But Jeb’s owners, Pam and Kenneth Job, have filed a motion for DNA testing to be conducted on the dead dog, a Pomeranian named Vlad.

Vlad died Aug. 24, and his owner, St. Clair resident Christopher Sawa, says he saw Jeb standing over his dog’s body. Both dogs were inside his backyard.

St. Clair County Animal Control took possession of Jeb after that.

Vlad was found with severe bruising over both shoulders and a puncture wound on his right front leg. There was another deep wound found on his left side that penetrated his chest and broke two ribs, the Detroit Free Press reported.

vladThe veterinarian who examined Vlad said his injuries were consistent with being picked up and shaken by a larger animal.

Ed Marshall, the lawyer for the Jobs, is asking the judge to allow them time to have an independent lab test conducted on Vlad’s body — to see if traces of Jeb’s DNA can be found in his wounds.

A hearing on his motion is set for Monday.

The Jobs say Jeb is an unofficial service dog who helps Kenneth with a condition that causes his muscles to deteriorate.

They say Jeb is a gentle soul and that Vlad’s death could have been caused by a fox or coyote, both of which can be seen from time to time in the rural area in which they live.

A modern day Dr. Frankenstein?

A controversial neurosurgeon in Italy said this week that he and his fellow researchers may be able to conduct the first human head transplant next year.

We suggest they start with their own.

Dr. Sergio Canavero has been compared to Dr. Frankenstein, and called a nut, but that hasn’t stopped him and members of his consortium — from China, South Korea and the U.S. — from severing the spinal cord of the beagle above (just so they could try to reattach it) and doing the same with numerous mice.

If that’s not weird enough, Canavero and team say that before they attempt a head transplant on a live human, they will conduct some experiments on human corpses, and then reanimate them with electricity to test his technique.

We can only assume they will do so in the basement laboratory of a castle, during a thunderstorm.

canaveroCanavero is director of the Turin Advanced Neuromodulation Group. He released three papers this week, and the video above, showing how he and his collaborators had successfully reattached the spinal cords of the dog and several mice.

Canavero also claims that researchers led by Xiaoping Ren at Harbin Medical University have already performed a head transplant on a monkey – connecting up the blood supply between the head and the new body.

Canavero’s short term goal is to successfully transplant a human head. His long term goal, he admits, “is immortality.”

What’s an acceptable number of dogs to torture in a quest of that nature?

We’d say none.

Canavero says the experiments on animals prove the technique used — known as GEMINI spinal cord fusion — incorporates a chemical called polyethylene glycol, or PEG, to encourage neurons to grow toward each other and connect.

He suspects it will also work in humans to fuse two ends of a spinal cord together, or to connect a transplanted head to a donor body.

He made the claims in a series of papers published in the journal Surgical Neurology International.

The claims have been met with widespread skepticism, according to New Scientist.

Canavero first announced his plans to conduct a human head transplant in 2013 and established the ead Anastomosis Venture, or HEAVEN, project to develop the techniques needed to carry out such an operation.

His collaborator in South Korea is Dr. C-Yoon Kim, a neurosurgeon at Konkuk University in Seoul who partially severed and reattached the spinal cords of 16 mice. Five of the eight mice who received PEG regained some ability to move. The other three died — as did eight who were in a control group.

In another experiment the South Korean team nearly severed the spinal cord of a dog. While the dog was initially paralyzed, three days later the team reported it was able to move its limbs and wag its tail.

South Korea is also the birthplace of dog cloning and up until this summer — when an American company cloned a dog for a customer — it was the only country cloning dogs for profit.

It’s probably not too outlandish — given all the bizarre turns medical researchers are taking — to wonder if surplus canine clones in South Korea end up being used for other wacky experiments by mad (or at least overly zealous) scientists.

In fact, if you look at its history, creating dogs for medical research use was one markets mentioned by the developers and marketers of dog cloning.

Could it be that some of the ideas initially presented in science fiction might ought to remain in the realm of science fiction?

Canavero’s research papers don’t indicate how many more dogs might have their necks snapped or heads severed by his research team as they boldly and single-mindedly stride toward their goal.

But, again, we’d argue that — no matter what medical gains it could lead to for humans — it should be NONE.

Dog clones: Now made in America

nubia2

Just as the earliest efforts to clone a dog in America didn’t make a huge splash, news-wise, neither did the recent birth — nearly 20 years later — of the first made-in-America canine clone.

ViaGen, a genetic preservation company in Texas, announced at the end of July that the first successful cloning of a dog in America had led to a birth, and that the Jack Russell terrier pup had been delivered to clients.

Chances are you haven’t read about it — because hardly anyone has written about it.

Including me — the guy who wrote that dog cloning book.

I received an email Monday containing the press release announcing the successful cloning. It came from Andrew Lavin, a public relations consultant in New York who handles publicity for ViaGen. It was dated Sept. 12 and included the photos of the clone, named Nubia, that you see here.

When I checked online to see what news coverage the announcement had received, I found almost none — only an “article” in Pet Age magazine (actually a verbatim reprint of the company press release) in July.

When I called ViaGen’s Austin offices to clear up some of my confusion I was told the press release had originally been issued at the end of July, and they didn’t know why the one I received had been re-dated to Sept. 12.

When I asked why the announcement had not received greater news coverage, the person on the phone said only, “It was a soft press release.” She didn’t explain what that meant.

(I can only guess it means a press release sent to a limited few, vague and fuzzy on the details, and accompanied by a “we’re not going to answer any questions” attitude — one that is low-profile enough to not arouse any detractors, such as the many animal welfare organizations that frown on cloning pets, saying it is cruel to animals and exploits bereaved pet owners.)

When I asked ViaGen for more information about the cloning, I was told, “all media requests go through Andy,” meaning Andrew Lavin.

He eventually returned my call and answered my email, explaining that he had “updated” the original press release — and therefore changed the date on it.

He did seek answers to my questions and sent me ViaGen CEO Blake Russell’s responses to them. Russell sidestepped far more than he answered.

nubia1The owners of the clone are not being identified — apparently not even the state or country where they reside.

Their original dog is deceased, but they were able to have her cloned with tissue samples taken by her vet when she was spayed.

Asked where the other dogs that are needed to produce a successful clone came from — dogs in heat from whom egg cells are harvested, and female dogs who serve as surrogates — Russell said ViaGen Pets purchases oocytes from an unnamed provider and that “ViaGen Pets uses a production partner to supply the needed surrogates.”

Presumably, the merging of egg and donor cells and the surgeries necessary were performed at ViaGen labs in Texas.

Texas, by the way, is where the whole crazy idea got started — though it wasn’t pulled off until scientists in South Korea cloned the world’s first dog.

Here’s the condensed version:

Shortly after the birth of the world’s first cloned mammal, Dolly the sheep, in 1996, the wealthy founder of the University of Phoenix, John Sperling, decided that cloning his girlfriend’s dog, Missy, would make for a lovely gift.

He teamed up with his girlfriend’s son, Lou Hawthorne, to find a learning institution that would be interested in cloning the world’s first dog.

They chose Texas A&M University and funneled millions into the project.

For years, from 1998 to 2002, researchers there tried to clone a dog. They were able to clone the world’s first pig, cat, bull and goat, but dogs, they found, were extra difficult.

Hawthorne had high hopes of turning the cloning of pet dogs into a big business, and it was during this time that he launched Genetic Savings & Clone, a company that, like Viagen, stored the cells of pets whose owners thought they might someday want a clone.

Snuppy

Snuppy

The research project at Texas A&M, eventually, was dropped, but the quest was picked up by Seoul National University in South Korea, which produced the first dog clone, Snuppy, in 2005.

The thousands produced since then — most often for bereaved pet owners seeking a duplicate of the dog they lost — have all been made in South Korean laboratories.

At one point, two Korean companies were producing dog clones for customers, and one American company was selling dog cloning, too.

Bio Arts, a company Hawthorne started in hopes of cloning dogs on its own, ended up teaming up with one of the Korean companies, Sooam, led by former Seoul National University scientist Hwang Woo Suk, to provide clones to American customers.

Among the first of those shipped back to the U.S. was a clone of Missy, which he presented to his mother, Sperling’s girlfriend.

She noted the puppy was ill-behaved, and said she didn’t want it.

SONY DSC

Surgery at Sooam

Hawthorne later pulled out of the partnership with Sooam, citing, among other reasons, his concerns that accepted animal welfare protocols — or at least those accepted by most Western countries — weren’t being followed by the South Koreans.

“A cloned dog contributes to the happiness of a family but I do not think it is possible to do it without a huge amount of suffering to hundreds of others,” Hawthorne told The Mirror, which was reporting on the first dog cloning for a customer in the UK.

In an interview with the Mirror, Hawthorne referred to the vast numbers of dogs that it took — up to 80, he said — to clone just one. And he confirmed that, as my book reported, Korean cloning researchers borrowed dogs from dog farms — farms where dogs are raised for their meat — for the process.

Today, only one of the Korean companies is still in operation.

https://www.amazon.com/Dog-Inc-Uncanny-Inside-Cloning/dp/1583333916Another Korean company that paved the way for cloning pet dogs — and provided the first clones to an American customer — pulled out of cloning pet dogs in 2011, not long after the publication of my book, “DOG, INC: The Uncanny Inside Story of Cloning Man’s Best Friend.”

ViaGen’s successful cloning of a dog lessens the likelihood of dog cloning fading away; instead it brings the process to American shores, and offers it at a much reduced price — $50,000 instead of the initial $150,000 the Korean companies charged.

ViaGen Pets says it is now the only American company offering pet cloning services — and says they are doing so “in full compliance with all U.S. regulatory standards and humane pet care practices.”

The are no federal laws against cloning dogs, or for that matter, humans, in the United States.

ViaGen,a long-time cloner of livestock, produced its first cloned cats for customers last year and it has been banking the cells of pets for more than a decade.

The company says the birth of Nubia will likely increase demand for cloning and genetic preservation of companion pet DNA.

screencapture-viagenpets-1473861354711

President Blake Russell said the company has already genetically preserved almost 1,000 pets and that there is a waiting list for the cloning procedure.

“The potential to have an identical twin to something that was very important and special in your life is an unprecedented opportunity and has brought a lot of joy to pet owners,” Russell says in the press release.

In addition to the cost of cloning, ViaGen charges a $1,600 fee and $150 a year to store tissue samples from pets whose owners may someday want to clone them.

The cloning procedure involves injecting cells harvested from the original dog into egg cells harvested from female dogs, a jolt of electricity to help them merge, and implanting the resulting embryo into a surrogate mother dog who carries the pup to birth.

ViaGen says a cloned puppy or kitten is “simply a genetic twin born at a later date, and should share many of the original’s attributes, including intelligence, temperament and appearance.”

The South Korean company guarantees only that the appearance will be identical, or nearly identical — but they often achieve that by producing multiple clones.

Many of dog cloning’s customers have come from the U.S. and the U.K. — and up to now they have been turning to Sooam Biotech to clone their dogs.

Most animal welfare organizations oppose the practice, pointing to the number of other dogs it takes to produce a clone, the intrusive procedures, the creation of surplus clones, and the sometimes nightmarish results. They also say pet cloning companies are exploiting the grief of bereaved pet owners.

There has been little outcry from them about the fact that dog cloning is now being done in America. Then again, it’s a development of which many people — possibly having missed that “soft” press release — aren’t aware.

In any case, it appears an American-born idea has finally — for better or worse — come to fruition in America.

(Photos of Nubia courtesy of ViaGen Pets; photos of Snuppy and a cloning underway at Sooam by John Woestendiek)

Identical twin dogs born in South Africa

twins

They’re being called the first identical twin dogs in history, which isn’t really true.

They’re being called the first “confirmed” or “recorded” identical twin dogs in history, which technically isn’t true either.

Not to be too nitpicky, and not to rain on anyone’s parade, but the first confirmed twin canine was born in 2005, created by man in a laboratory, with help from a few jolts of electricity.

He was an Afghan hound, named Snuppy. And his twin was the donor dog, whose extracted cells he emerged from. Thousands of identical twins have been born since then. They are called clones.

So to be annoyingly accurate, we must call the Irish Wolfhound brothers born in South Africa earlier this year the first confirmed and recorded identical twin dogs that aren’t clones.

twinfamily1They were delivered by Kurt de Cramer, a veterinarian in South Africa’s Rant en Dal Animal Hospital in Mogale City, who, during a Caesarean section, was surprised to find two puppies in the same placenta.

“When I realizd that the puppies were of the same gender and that they had very similar markings, I also immediately suspected that they might be identical twins having originated from the splitting of an embryo,” de Cramer. told the BBC.

The significance of that is that — though dogs from the same litter often look alike — it has never been documented before.

de Cramer called upon colleagues to help confirm the finding. The team, including Carolynne Joone of James Cook University in Townsville, Australia and Johan Nöthling of the University of Pretoria in South Africa, obtained blood samples when the twins were two weeks old.

Those tests, and subsequent ones on tissues six months later, showed their DNA to be identical,

Their findings were published in the journal Reproduction in Domestic Animals.

While it is the first case of its type to be recorded in scientific literature, the birth of identical twin dogs may not be all that rare.

Pups in a litter often look similar. DNA tests are not routinely performed. And because mother dogs generally eat (or if you prefer, clean up) the placenta after birth, evidence of two dogs sharing a placenta doesn’t linger.

Twins can be either monozygotic (identical), meaning they develop from the same zygote (or egg cell), which is fertilized by the same sperm cell; or they can be dizygotic (fraternal), meaning they develop from two different egg cells, each fertilized by separate sperm cells.

Twinning in mammals is uncommon, occurring regularly only in humans and armadillos. While it has been reported in horses and pigs before, both twins rarely survive.

Today the twin dogs, called Cullen and Romulus, are doing well. They were slightly smaller than normal at birth, but by six weeks of age they had reached a similar size to the other pups in their litter.

Cute as they are, Cullen and Romulus are not really trailblazers. Most likely, many identical twin dogs have been born over the years — the natural way — and gone undetected.

For sure, hundreds more have been born in recent years the grossly unnatural way.

So, sorry about that nature, but when it comes to the “first” identical twin dogs — at least according to the written record, and the “scientific literature” — technology beat you to the punch.

(Photos: Kurt de Cramer, via BBC)

It’s what you say AND how you say it

enik kubinyi

Traditional wisdom holds that it’s not so much what you say to your dog as how you say it that counts — that tone, in other words, is everything.

But scientists in Hungary say dogs may understand more words than we think — and that it takes a combination of positive words and a positive tone for their brains to register a pleasurable reaction.

“Both what we say and how we say it matters to dogs,” said Attila Andics, a research fellow at Eotvos Lorand University in Budapest.

MRI readings conducted in the study showed the right hemisphere of dogs’ brains react to intonation, while the left hemisphere reacts to the meaning of words — as is the case with humans.

Their paper was published in this week’s issue of the journal Science.

The researchers — using words, positive tones and plenty of treats, we’d imagine — trained dogs to enter a magnetic resonance imaging machine and lie still while the machine recorded their brain activity.

The methods, similar to those being used at Emory University, are allowing scientists to better understand what goes on in the canine brain.

enik kubinyi2A trainer spoke common words of praise used by dog owners, including the Hungarian words for “good boy,” “super” and “well done,” as well as neutral words like “however” and “nevertheless.”

All the words were spoken using both positive tones and neutral tones, according to the New York Times.

Only words of praise spoken in a positive tone provoked significant reactions, making the reward centers in a dog’s brain light up.

The researchers at Eötvös Loránd University in Budapest recruited 13 family dogs for the study, and trained them to sit totally still for seven minutes in an fMRI scanner. The dogs were not restrained, and “could leave the scanner at any time,” the authors said.

Using the brain activity images, the researchers saw that the dogs processed the familiar words regardless of intonation, and they did so using the left hemisphere, just like humans. Tone, on the other hand, was analyzed in the auditory regions of the right hemisphere.

Using neutral words in a positive tone, or positive words in a neutral tone, produced little reaction — or at least not one that shows up in MRI machines.

“It shows that for dogs a nice praise can very well work as a reward, but it works best if both words and intonation match,” Andics said. “So dogs not only tell apart what we say and how we say it, but they can also combine the two, for a correct interpretation of what those words really meant.”

(Photos by Enik Kubinya, via New York Times)

Which motivates more — food or praise?

kadypraise

A study at Emory University suggests that dogs aren’t strictly the food-obsessed beasts they’ve traditionally been seen as — and that many, maybe even most, prefer attention and praise over a chewy treat.

While only 13 dogs participated in the study, there were only two of them who — judging from their neural reactions — showed a distinct preference for food over praise.

The study, published in the journal Social, Cognitive and Affective Neuroscience, is one of the first to combine brain-imaging data with behavioral experiments to explore what kind of rewards canines prefer.

“We are trying to understand the basis of the dog-human bond and whether it’s mainly about food, or about the relationship itself,” says Gregory Berns, a neuroscientist at Emory and lead author of the research.

“Out of the 13 dogs that completed the study, we found that most of them either preferred praise from their owners over food, or they appeared to like both equally. Only two of the dogs were real chowhounds, showing a strong preference for the food.”

Berns heads the Dog Project in Emory’s Department of Psychology. It was the first to train dogs to voluntarily enter a functional magnetic resonance imaging (fMRI) scanner and remain motionless during scanning, without restraint or sedation.

Their previous research using the technique identified the ventral caudate region of the canine brain as a reward center and showed that region responds more strongly to the scents of familiar humans than to the scents of other humans, or even to those of familiar dogs.

Phys.org reports that, in the new study, researchers trained the dogs to associate three different objects with different outcomes. A pink toy truck signaled a food reward; a blue toy knight signaled verbal praise from the owner; and a hairbrush signaled no reward, to serve as a control.

The dogs then were tested on the three objects while in an fMRI machine. Each dog underwent 32 trials for each of the three objects as their neural activity was recorded.

Four of the dogs showed a particularly strong activation for the stimulus that signaled praise from their owners. Nine of the dogs showed similar neural activation for both the praise stimulus and the food stimulus. And two of the dogs consistently showed more activation when shown the stimulus for food.

Berns says the findings run counter to the old view that dogs “just want food and their owners are simply the means to get it … Another, more current, view of their behavior is that dogs value human contact in and of itself.”

In another part of the study, dogs were put into a Y-shaped maze in which one path led to a bowl of food and the other path to the dog’s owner.

The dogs were repeatedly released into the room and allowed to choose one of the paths.

While most dogs alternated between the food and their owner, dogs who showed a greater response to praise in the first part of experiment chose to go to their owners 80 to 90 percent of the time.

Berns said the study “shows the importance of social reward and praise to dogs. It may be analogous to how we humans feel when someone praises us.”

ozziefood

(Photos: At top, Kady, a Lab-retriever mix in the study who preferred praise from her owner to food; at bottom, Ozzie, a shorthaired terrier mix who chose food over his owner’s praise / Emory University)

“Erect-tail dysfunction:” Losing the wag

erecttaillab

The university that cloned the first mammal is now investigating why some older dogs — especially those in colder climes — sometimes experience limp tails.

A study at Edinburgh University in Scotland says the phenomenon known as ‘limber tail,’ which causes a dog’s tail to become limp and difficult to move, tends to affect larger working breeds, and is more common among dogs who live in the north.

The study of dozens of dogs found that the chance of a dog developing the condition rose by 50 per cent for each additional degree of latitude further north he or she lived.

Working dogs, who spend more time outdoors, and those who enjoy swimming were also around five times more likely to develop “limber tail,” which is also known as “cold tail” or “swimmer’s tail.” More informally, the condition is sometimes referred to as a dog “losing their wag.”

Personally, we like the name a Telegraph headline writer gave the condition: Erect-tail dysfunction.

No, this is not The Onion you’re reading, and this is not a joke — at least not to the dogs who get it. Owners report that it can be very painful and distressing for the dogs.

“We were surprised by how many owners were reporting limber tail to us but it meant we had the chance to do a detailed investigation,” said Dr. Carys Pugh, of The Roslin Institute and Royal Dick School of Veterinary Studies.

The Royal Dick School of Veterinary Studies, commonly referred to as the Dick Vet, is no joke, either. It’s the veterinary school of the University of Edinburgh.

Dolly the sheep, the world’s first mammal clone, was born at the Roslin Institute in 1996, marking a new era of biological control. Nine years later the cloning of dog — the 18th species successfully cloned — was achieved by scientists in South Korea.

limptaillabIn the limber tail research, a team at the University of Edinburgh compared 38 cases of dogs with limber tail with 86 dogs that had no symptoms.

Those affected by limber tail were more likely to be working dogs, and more likely to regularly swim.

The condition, which was first reported in the scientific press in 1997, is thought to affect around 60,000 dogs in Britain, “but owners often struggle to find out what is wrong with their pets as there is little literature available,” the Telegraph reported.

It’s generally not a lifelong condition; rather, it resolves itself within a few days or weeks.

The researchers hope further studies will identify genes associated with the condition, which could one day help breeders to identify animals that are likely to be affected.

Caroline Kisko, Secretary of Kennel Club, which funded the research through its charitable trust, said owners should be careful not to over-expose their dogs to the cold.

“The condition is rare, but is it most often seen in working dogs such as Labrador retrievers, flat coated retrievers and pointers. Dogs usually recover their normal tail posture and function over a period of days or weeks, however it can be painful.”

Gudrun Ravetz, junior vice president of the British Veterinary Association warned owners not to become so worried about the cold that they stop exercising their pets.

“Limber tail is rarely seen in veterinary practices and the research indicates that most owners do not seek veterinary attention for this problem,” she said.

(Photos: Top, a young chocolate Lab with a perky and lively tail; bottom, an older chocolate Lab whose tail has gone limber)