Ten years after a dog was first successfully cloned, scientists have managed to produce the world’s first litter of pups to be born through in vitro fertilization.
In July seven puppies were born through IVF at Cornell University — five beagles and two “bockers,” or beagle-cocker spaniel mixes.
The achievement was not revealed until this week with the release of the research study.
Seems like science would have happened the other way around — that a “test-tube” puppy would have premiered long before we entered the even more science fiction-like era of cloned dogs becoming available on the marketplace.
But, while IVF has been used for decades in other animals, including humans, scientists had never succeeded in using it to produce a newborn pup.
Previous attempts to use IVF in dogs had resulted in very low rates of fertilization, and no live births at all once IVF embryos were transferred to a host.
“Since the mid-1970s, people have been trying to do [IVF] in a dog and have been unsuccessful,” said co-author Alex Travis, associate professor of reproductive biology at the Baker Institute for Animal Health in Cornell’s College of Veterinary Medicine.
What made it so difficult were some of the same factors that proved challenging in cloning dogs — females only ovulate once or twice a year, and their eggs are not transparent, making it harder to see the structures inside of the egg.
The Cornell researchers, in a joint project with researchers from the Smithsonian Institution, found that by waiting an extra day for eggs to mature before extracting them, they met with more success.
Adding magnesium to the environment where the sperm and egg met also helped with fertilization, the team found, according to a Cornell press release.
The achievement was revealed this week in a study published online Dec. 9 in the journal Public Library of Science ONE.
The seven surviving puppies (out of 19 embryos) are genetically the offspring of two different fathers (a cocker spaniel and a beagle) and three different beagle mothers, carried by the same beagle surrogate.
Unlike cloning, which involves transferring an existing (or dead) dog’s DNA into a donor egg, IVF involves the creation of a new genome through fertilization. Each each animal has a unique set of DNA.
The researchers say the development will open the door for preserving endangered canid species using assisted reproduction techniques.
It could also enable researchers to eradicate heritable diseases in dogs and facilitate the study of genetic diseases in dogs and humans, they say.
(Photo: Cornell graduate student Jennifer Nagashima and Smithsonian Conservation Biology Institute research biologist Nucharin Songsasen — lead author and co-author of the study — walk some of the puppies born through IVF; by Jeffrey MacMillan / Cornell University)
Posted by John Woestendiek December 10th, 2015 under Muttsblog.
Tags: animals, assisted, beagles, biology, bockers, cloned, clones, cloning, cocker spaniels, cornell, cornell university, dna, dog, dogs, egg, first, in vitro, in vitro fertilization, ivf, pets, puppies, pups, reproduction, research, science, sperm, study
At age 3, his owners in Minnesota figured Beau — full name Victoire Gerie’s No Lemon Gemstone — could breed at least until he was 10.
In the process, they figured, they would be ensuring his genes and his legacy lived on .
And they’d get the puppy that they desperately wanted.
But those hopes, and those bucks, seemingly became a thing of the past when Beau’s breeder had the little white dog neutered without their knowledge, owners Mary and John Wangsness allege in a lawsuit.
The legal dispute has been going on for about a year now in Minnesota’s Ramsey County, according to the Minneapolis Star Tribune.
The Wangsnesses allege breeder Vickie Halstead, who sold them the dog, acted in “vengeance” by neutering Beau because they had tried to breed him twice to a female dog, Cha Cha, without obtaining Halstead’s approval, which was required in the sales contract.
They are seeking more than $50,000 in damages, and about eight vials of what they believe to be Beau’s frozen semen, now stored in a veterinary clinic and estimated to be worth $3,000 each.
The semen is being held under Halstead’s name, and the lawsuit alleges she has already profited from selling two vials.
As John Wangsness sees it, since it came from the loins of his dog, what’s in those vials are his.
“Damn right, they’re mine,” he said.
Beau was neutered without their approval in July 2013, when he was 4.
“After hearing about the neutering, and I’m not overstating things at all, Mary literally cried and stayed in bed for three weeks,” said Wangsness, adding that she never fully recovered before she died this past March.
The case isn’t as black and white as it might seen. In the competitive world of dog showing, ownership of a dog — as well as decisions about its care and profits — are often contractually shared between the breeder and the owner.
And that much debated sperm might not even be Beau Lemon’s.
Halstead’s attorney, Joseph Crosby, said at a recent hearing that the frozen semen belongs to Beau’s brother, Beau Jangles.
Crosby said Halstead “rescued” the dog from the Wangsnesses because they were neglecting him. He said Beau was suffering from dental disease, a low sperm count, impacted anal glands, and a matted and unhealthy coat.
Crosby said Beau’s neutering was necessary due to his “deteriorated health condition.”
In June of 2013, Halstead borrowed Beau from the Wangsnesses for what she told them was breeding purposes, the lawsuit says.
They did not learn of his neutering until he was returned.
Larry Leventhal, attorney for the Wangsnesses, said the couple treated Beau as a pet, but they also expected to have the option of breeding him several times a year at a rate of $2,000 to $3,000 per breeding until he turned 10.
Wangsness said that, more than money, he wants justice for his wife.
“I would like some vindication for the emotional distress that happened to Mary as a result of [Beau’s neutering],” Wangsness said.
Attorneys were scheduled to meet Tuesday to discuss a settlement agreement.
(Photo: Beau, as pictured on the website for Victoir’s Bichons)
Posted by John Woestendiek September 23rd, 2015 under Muttsblog.
Tags: animals, beau lemon, bichon frise, breeders, breeding, champion, competition, court, dog, dog shows, dogs, frozen semen, lawsuit, neutered, pets, Ramsey County, settlement, sperm, Vicki Halstead
It’s a well-known but little publicized fact that some dogs competing at Westminster and in many other dog shows aren’t brought into the world in a 100 percent natural way.
Since the 1960s, breeders have been harvesting semen from male purebreds — one technique for which is demonstrated in the video above — and inserting it into females in hopes of creating champions.
The American Kennel Club, though it doesn’t allow cloned dogs to participate in the dog shows it sanctions, has no problem with permitting those who are products of artificial insemination.
Over the decades, as with artificial insemination in humans, the technology has progressed and become widely accepted. (My view is, if we are going to widely accept something, we shouldn’t balk at watching it.)
While some human, uh, effort is involved in the semen-gathering method depicted above, more state of the art techniques involve artificial vaginas and electro-stimulation. Even today though, to get the canine juices flowing, breeders commonly use a female dog in heat, parading her in front of the male. She’s referred to as a “teaser bitch.”
Breeders say practicing artificial insemination can help improve the quality of breeds. For sure, it gives them more control, allowing them to overcome logistical obstacles, such as when a male and female are living on opposite ends of the country. They can still have a long distance relationship, so to speak.
It allows a champion male to breed with many more females than would be physically possible through traditional one-on-one mating. It allows older male dogs to continue reproducing after they can no longer mount a female. And it allows a male dog to keep producing offspring long after his death, which is the case with a champion Old English sheep dog named Yoshi.
Yoshi, under his registered name, Lambluv Desert Dancer, won more best in shows than any other sheep dog. He won Best of Breed at Westminster three times, most recently in 1999. He died in 2006, but he could still be daddy to more than 100 future litters.
“I have about 100 straws,” his owner Jere Marder told Bloomberg.com, in reference to the frozen semen samples from Yoshi she has in storage.
No product of artificial insemination has won at Westminster, but last year’s runner up in Best in Breed was a dog created with 17-year-old sperm from one of Lambluv’s sheep dogs.
“Most serious breeders that I know of have something in store,” says Marder, who owns Lambluv Old English Sheepdogs. “If anything, it’s just a precaution; otherwise, if anything happens to your champion dog before you can breed him, you’re out a good chunk of money.”
“It’s definitely a market — and one that’s growing,” said Randall Popkin, owner of the California-based Breeder’s Veterinary Services, which has been storing frozen semen and inseminating dogs with it since 1984.
“When I first started, few breeders were doing this,” he said. “Nowadays, you travel to dog shows and there’ll be three companies there offering to freeze your dog’s semen.”
According to the American Kennel Club, the number of registered purebred litters conceived with frozen semen has risen by 26 percent over the past decade. In 2013, the year for which the most recent data is available, the AKC registered about 2,200 litters that were produced via artificial insemination. That’s about 1 percent of all AKC-approved litters.
The Bloomberg article notes there are downsides.
In 2009, a Pembroke Welsh corgi breeder sued an animal hospital after her dog was allegedly accidentally inseminated with sperm from a Great Pyrenees — a breed roughly five times her dog’s size. The corgi nearly died giving birth.
In addition, there have been lawsuits over samples that were damaged during shipping or produced puppies that didn’t look purebred. In 2012, a jury awarded $200,000 to a Pennsylvania breeder who had sued a veterinary hospital for accidentally defrosting more than 100 samples from her champion poodles.
Marder, who sat out Westminster this year, says she’d love to see one of dead Yoshi’s offspring win there someday. Doing so, the article said “feels to her as if she’s keeping her old dogs alive.”
(Photo: Yoshi, from the website Lambluv.com)
Posted by John Woestendiek February 19th, 2015 under Muttsblog, videos.
Tags: animals, artificial insemination, artificial vagina, breeders, breeding, breeds, dog shows, dogs, harvesting, pets, purebreds, semen, sheep dog, sheepdog, sperm, teaser bitch, westminster, yoshi
When Karen and Anthony Scully filed for divorce, determining custody of the six dogs wasn’t too difficult. He got four, she got two. The sticking point came later — with a feud, still going on five years later, over who should get the dogs’ semen.
Karen Scully, who lives in Florida, and her ex-husband, Anthony Scully of Oakland County, Michigan, are feuding in Oakland County Circuit Court in Pontiac over who has the legal right to the semen belonging to Cyrus, Regg and Romeo, all AKC-registered bullmastiffs.
Bull mastiff pups fetch $2,000 each in the marketplace.
The Scullys were hobby breeders in Oakland County until their divorce in 2002, and they had banked sperm from their mastiffs in a freezing center in Sterling Heights. Both still raise bullmastiffs.
Anthony Scully, through his attorney, said the semen is his — or rather, is property that should belong to him — and that his ex-wife, in moving to Florida, gave up claim. Karen Scully, who appeared in court via teleconference, claimed she has ownership, since the dogs that provided the semen once belonged to her.
Judge Matthews said she thought the case was a prank at first. “I asked, ‘Am I Being Punk’d … Is this a Candid Camera thing?” she said.
Matthews ruled that it was not a divorce matter and told the couple they would have to fight it out in civil court, according to an article in the Detroit Free Press.
The case has been assigned to Oakland County Circuit Judge Leo Bowman.
(Photo: Anthony Scully with Romeo, family photo)
Posted by John Woestendiek April 10th, 2009 under Muttsblog.
Tags: animals, anthony scully, breeders, breeding, bull mastiff, court, custody, divorce, divorced, dog, dogs, freeze, freezing, frozen, karen scully, mastiff, michigan, pets, pontiac, semen, sperm